Radiosensitivities in various DNA repair deficient CHO cells irradiated with heavy charged particles

Abstract

To investigate the possible mechanisms of DNA damage repair in complex DNA aberrations produced by heavy charged particles, we demonstrated the experiments that various DNA repair deficient CHO cells were irradiated with X-ray and heavy charged particles (Carbon ions 290MeV/n LET13 or 70keV/micrometer, Iron ions 500MeV/n LET 200keV/micrometer) or treated with chemical agents, mitomycin C or cisplatin. DNA repair mutant CHO cell lines that we used in this study were V3 (DNA-PKcs deficient), xrs5 (Ku80 deficient), 51.D1 (rad51D mutant), and KO40 (fancg mutant). Asynchronous cell population was irradiated and immediately re-plated for colony formation assay for testing radio- or chemical sensitivities. Results will be presented in the meeting.