Host: The Japan Radiation Research Society
In this study, the effect of quantum ray irradiation product (bovine serum albumin which irradiated gamma-ray at 5 kGy) on DNA synthesis was examined. The mutation rate of DNA synthesis has determined for a single round of gap-filling reaction using M13mp18 DNA as a primer-template. The mutation rate was measured using the colored selection method. Then, the characteristics of mutation were examined using the sequence analysis.
The result shows that the mutation frequencies were 7.35×10-5 for the control and 8.40×10-4 in the presence of quantum ray irradiation product.
Mutation sites were determined by DNA sequencing. We sequenced 27 randomly chosen LacZ mutant plaques. The results show that the substitution mutations were located at the position 91 on the plus strand of LacZ gene, and that the mutation occurred next to homopolymeric runs.
