Abstract
Recently, a lot of experimental model organisms have been developed. They have the advantage that genetics can be used full. Medaka is most suitable because both of classical and modern molecular genetics are available. Recently a general reverse genetics method was reported, which can identify mutations in genes that are known only by their sequence. The method, called TILLING (Targeting Induced Local Lesions IN Genome), includes random mutagenesis, followed by screening for induced mutations in target genes at the genomic DNA level. Adult male Medaka were mutagenized with ENU and then outcrossed with female to generate F1 progeny for the library. We established about 5,700 ENU-mutagenized F1 male fishes. To construct a library, genomic DNA and testis samples were isolated and cryopreserved from each F1 fish. And we established screening methods. Screening revealed that the average per-base mutation frequency was 1 in 300kbp recently. The thing indicated that can be used enough for screening, and some nonsense mutants have already been obtained. We have introduces the outline of the entire screening including detect of the mutant.
