Abstract
SMG1; PI-3 kinase and UPF1; RNA helicase are well known for their critical role in Nonsense Mediated mRNA Decay (NMD) pathway, which causes the elimination of mRNAs containing premature stop codons. However, their essential role in DNA metabolism (cell cycle progression and DNA damage sensoring) has been also demonstrated. Their function in DNA metabolism appears to be independent of NMD and SMG-1 plays important roles in the maintenance of both genome and transcriptome integrity in human cells. In this study, we analyzed the role of SMG1 on the expression p53 by RNAi. Accumulation of p53 by proteasome inhibitors reduced in SMG1 knock down cells and this seems to imply that SMG1 regulates the amount of p53.
