Abstract
Excessive oxidative stresses attack a wide variety of cellular important molecules, including lipids, protein, and DNA, resulting in damage that compromises cell integrity and function. It is known that there is clear difference in immortalization and carcinogenesis between human and rodent cells. These suggest that difference of immortalization ability and carcinogenesis sensitivity within creature species depends on its oxidative stress control mechanism. Therefore this study was carried out to compare the difference of response to oxidative stress and genetic changes in immortalization and carcinogenesis with human cells and rodent embryonic cells cultured under atmospheric oxygen (20%) and hypoxic (2% and 0.5%) conditions.
As a result, human cells never made immortal or acquire anchorage independent growth under all the oxygen pressure. On the other hand, in hypoxically-cultured rodent cells, susceptibly response to oxygen pressure and spontaneous chromosomal aberrations were observed at high frequency. Additionally, rodent cells immortalized under all the oxygen pressure and cells cultured under 2 and 20% oxygen tensions got anchorage independent growth ability after having acquired unlimited lifespan.
These results suggested that human cells metabolize oxygen or maintain the homeostasis superior than rodent cells, and contributes to suppress the cell immortalization or carcinogenesis
We will present the relation of cell immortalization and oxygen sensitivity.
