DMSOによる放射線防護効果はDNA-PK依存的修復に依存する

菓子野 元郎; 漆原 あゆみ; 児玉 靖司; 小林 純也; 劉 勇; 鈴木 実; 増永 慎一郎; 木梨 友子; 渡邉 正己; 小野 公二

doi:10.11513/jrrsabst.2008.0.209.0

The 51st Annual Meeting of The Japan Radiation Research Society

Session ID : EO-1-3

DOI https://doi.org/10.11513/jrrsabst.2008.0.209.0

Conference information

Host: The Japan Radiation Research Society

Co-host: City of Kitakyushu, University of Occupational and Environmental Health, Japan

Radiation therapy / Modifing Factors

Radioprotective effect by DMSO is dependent on DSB repair through the DNA-PK pathway

*Genro KASHINO, Ayumi URUSHIBARA, Seiji KODAMA, Junya KOBAYASHI, Yong LIU, Minoru SUZUKI, Shin-Ichiro MASUNAGA, Yuko KINASHI, Masami WATANABE, Koji ONO

Author information

*Genro KASHINO
Particle Radiation Oncology Research Center, Research Reactor Institute, Kyoto University
Ayumi URUSHIBARA
The Nippon Dental University School of Life Dentistry at Tokyo
Seiji KODAMA
Frontier Science Innovation Center, Organization for Industry, University and Goverment Coorperation, Osaka Prefecture University
Junya KOBAYASHI
Radiation Biology Center, Kyoto University
Yong LIU
Particle Radiation Oncology Research Center, Research Reactor Institute, Kyoto University
Minoru SUZUKI
Particle Radiation Oncology Research Center, Research Reactor Institute, Kyoto University
Shin-Ichiro MASUNAGA
Particle Radiation Oncology Research Center, Research Reactor Institute, Kyoto University
Yuko KINASHI
Laboratory of Radiation Safety and Control, Research Reactor Institute, Kyoto University
Masami WATANABE
Laboratory of Radiation Biology, Research Reactor Institute, Kyoto University
Koji ONO
Particle Radiation Oncology Research Center, Research Reactor Institute, Kyoto University

Keywords: dimethyl sulfoxide, Radioprotective effect, DNA-PK

CONFERENCE PROCEEDINGS FREE ACCESS

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Abstract

We examined the mechanism for the radioprotective effect by DMSO treatment. When CB09 mice cells were treated with 2%DMSO 1 h before and during X-irradiation, radioprotective effects were clearly observed in survival assay and micronucleus assay. The suppressive effects of DSB by DMSO were also observed both 15 min and 2 h after irradiation. Interestingly, the ratio of suppression by DMSO in 2h after irradiation was higher than that in 15 min after irradiation. This result suggests that DSB repair is activated between 15 min and 2 h after irradiation by DMSO treatment. Moreover, little protective effects by DMSO were observed in DNA-PKcs deficient SD01 cells derived from scid mice. These results suggest that the radioprotective effect by DMSO is brought by activation of DNA-PK dependent repair, not by suppression of indirect action.

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