Bystander effect observed in mouse splenocytes following cranial gamma-ray irradiation

Abstract

Bystander effect observed in mouse splenocytes following cranial gamma-ray irradiation Yuko Kinashi, Hiroki Tanaka, Shinichiro Masunaga, Minoru Suzuki, Genro Kashino, Liu Yong, Koji Ono, Sentaro Takahashi Research Reactor Institute, Kyoto University The evidence of bystander effect was inspected in vivo study following the local radiation. The head of C3H mice were exposed to 10Gy of gannma ray, while the other part of body was shielded with 5cm thick lead block. The effective dose for reminder body except head was calculated 1.0-1.4Gy. The splenocytes were isolated 1-24 hours after the cranial irradiation and the apoptosis was measured with Elisa kit (Roche). The T-lymphocytes were isolated and the micronucleus induction was estimated by the cytokinesis-block micronucleus assay. The damage of splenocytes measured by these above two method was more excessive than that is prospected under the radiation dose of 1.0-1.4Gy. Our results suggested that bystander effect was observed in distant non-irradiated organ under the local irradiation.