異形性グリオーマの放射線とヒ素による老化様細胞増殖停止の誘導に関する研究

二宮 康晴; 于 冬; 関根 絵美子; 平山 亮一; 野口 美穂; 加藤 宝光; 高橋 千太郎; 丹羽 太貫; 藤森 亮; 岡安 隆一

doi:10.11513/jrrsabst.2008.0.239.0

The 51st Annual Meeting of The Japan Radiation Research Society

Session ID : EP-12

DOI https://doi.org/10.11513/jrrsabst.2008.0.239.0

Conference information

Host: The Japan Radiation Research Society

Co-host: City of Kitakyushu, University of Occupational and Environmental Health, Japan

Radiation therapy / Modifing Factors

Analysis of induction of senescence-like growth arrest in glioblastoma cells by irradiation or arsenite.

*Yasuharu Ninomiya, Dong Yu, Eimiko Sekine, Ryoichi Hirayama, Miho Noguchi, Takamitsu Kato, Sentaro Takahashi, Ohtura Niwa, Akira Fujimori, ryuichi Okayasu

Author information

*Yasuharu Ninomiya
Research Center for Charged Particle Therapy, Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences
Dong Yu
Research Center for Charged Particle Therapy, Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences
Eimiko Sekine
Research Center for Charged Particle Therapy, Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences
Ryoichi Hirayama
Research Center for Charged Particle Therapy, Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences
Miho Noguchi
Advanced Science Research Center, JAEA
Takamitsu Kato
Research Center for Charged Particle Therapy, Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences
Sentaro Takahashi
Kyoto University Research Reactor Institute
Ohtura Niwa
Research Center for Charged Particle Therapy, Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences
Akira Fujimori
Research Center for Charged Particle Therapy, Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences
ryuichi Okayasu
Research Center for Charged Particle Therapy, Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences

Keywords: Radiosensitaization, Brain tumor, Senescence

CONFERENCE PROCEEDINGS FREE ACCESS

Details

Abstract

[Purpose] Gliomblastoma is one of the most common tumor types resistant to radiation. Arsenite was reported to show synergistic radiosensitization effect in vitro and in vivo. But, the mechanism of radiosensitization is not well understood. Last year, we shown the induction of senescence-like growth arrest (SLGA) including heterochomatin formation by treatment of radiation or arsenite. This year, we have studied the difference of mechanism of SLGA between radiation and arsenite. [Results] Glioblastoma U87MG cells treated by irradiation or arsenite. It is kown as p21 or p16 relate to SLGA. U87MG lacks the p16 gene. Thus, we analysed the p21 by Westen blotting. The significant induction of p21 was observed by irradiation, but not by treatment of arsenite. Now, p21 dependency about SLGA by irradiation or treatment of arsenite are analysing by siRNA and the data will be presented.

Corresponding author

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