Abstract
There are two pathways, in which DNA double strand breaks (DSBs) are rejoined by non-homologous recombination and homologous recombination (HR).Nijmegen breakage syndrome(NBS) is genetic disease characterized by ionizing radiation sensitivity and genome instability. NBS1, gene responsible for NBS, is reported that it is important factor for HR repair. NBS1 forms complex with Mre11 and Rad50 (MRN complex) and involved in sensor, signaling, and repair of DSBs. On the other hand, SbcC/SbcD/Xrs2 complex, homolog of the MRN complex, is also involved in a wide variety of DNA repair and has sensitivity to alkylating agent, suggesting the role of MRN complex in repair of base damage. Using NBS1 mutant cell line, we have examined whether NBS1 functions in a response to alkylating agents.
