Abstract
Aneuploidy—the presence of an abnormal number of chromosomes— occurs in ~90% of solid human tumors and ~75% of hematopoietic cancers and was recognized as a common characteristic of tumor cells 100 years ago. Although the role of aneuploidy in cancer has been at the center of debate for almost a century, whether it is a cause or a consequence of the malignant transformation (oncogenesis) has not been clear. So we examine the role of aneuploidy in tumorigeneous.
In this study, to determine the effects of an additional chromosome on diploid cell character that caused by spontaneousness, abnormality of centrosome, abnormality of chromosome segregation gene, or orther, we generated aneupolid cells that carried an additional chromosome. With immortalized human mesenchymal stem cells that carried an additional chromosome, we will report the result of mutation assay, anchorage-independent growth assay, micronuclei assay. And with nomal human cells, we will report the result of same assays.
