Host: The Japan Radiation Research Society, Chairman of the 52nd Annual Meeting, Toshiteru Okubo (Radiation Effects Research Foundation)
We used budding yeast S. cerevisiae and analyzed influence in it by two kinds of different radiations, a carbon beam and the proton beam. A survival rate was less than 10% by irradiation of 50Gy by the carbon beam and less than 10% by irradiation of 300Gy by the proton beam. Interestingly, a survival rate was not 0% even if we irradiated 2000Gy by the carbon beam. Furthermore, we compared the survived yeast by carbon beam irradiation of 2000Gy with a wild type strain, and a survival rate increased 8-10 times with a carbon beam of 300Gy, and a survival rate increased more when we irradiated once again. This result suggested that the yeast got radiation resistance. Therefore we performed single colony isolation to analyze specific having radiation resistance yeast or not. Concretely, we analyzed change of growth speed, high temperature sensitivity, low temperature sensitivity, MMS sensitivity, Bleomycin sensitivity and the change of the cell cycle by the checkpoint defect. In addition, we report a difference of the protein by the two-dimensional electrophoresis and a abnormality of the chromosome by a pulse field electrophoresis
