Host: The Japan Radiation Research Society, Chairman of the 52nd Annual Meeting, Toshiteru Okubo (Radiation Effects Research Foundation)
Purpose: Cell-mediated immunity decrease by radiation becomes known, but it is not studied mechanism of effect of humoral immunity in detail. Therefore, in this study, we were aimed at reviewing cell-mediated immunity by radiation, effect to humoral immunity by measuring
Methods: We classified in control group, 0.5Gy group, 1Gy group and 2Gy group. We measured a blood cell count (leukocyte, lymphocyte, monocyte, granulocyte) after 30th since the irradiation day before. We measured by IL-2 Kit, IL-2 Kit, IL-6 Kit, IL-12 Kit of mice serum.
Results: In lymphocytes, meaningful decrease was accepted in 0.5Gy group 24 hours later. In 1Gy group, meaningful decrease was accepted after 3hours, 24 hours and 3days later. In 2Gy group, meaningful decrease was accepted in all assay dates. In IL-2, it was control group comparison with the irradiation day before, it was it with 199.6% in a 0.5Gy crowd in 57.9%, 2Gy group in 425.6%, 1Gy group after irradiation 15th whereas it was 24.6%. In addition, it was it with 170.8% in a 0.5Gy crowd in 158.7%, 2Gy group in 383.8%, 1Gy group after irradiation 30th whereas appearing was 96.6% in control group. In IL-6, meaningful decrease in 0.5Gy group after 7th after 3rd 24 hours later. In 1Gy group, meaningful decrease was accepted after 30days and 7days later. In 2Gy group, meaningful decrease was accepted 24 hours later. In IL-12, meaningful decrease was accepted in 0.5Gy group after 30th. In 2Gy group, meaningful decrease was accepted after 30th after 15th three days later.
Conclusions: In this study, blood corpuscle ingredients such as lymphocytes are affected by effect of radiation early. Production tends to enhance IL-2 by radiation hormesis by radiation of a low dose of 0.5Gy. As for the IL-6, quantity of production decreases by effect of radiation. However, quantity of production of IL-6 tends to increase in 0.5Gy early. IL-12 is tendencies to decrease by radiation, but rather quantity of production tends to increase in low dose radiation. Humoral immunity tended to commit radiation in predominance than the above in the early stage when it was exposed to radiation, and it was accepted that cell-mediated immunity tended to act on predominance with time.
