Host: The Japan Radiation Research Society, Chairman of the 52nd Annual Meeting, Toshiteru Okubo (Radiation Effects Research Foundation)
Post-replication repair pathway (PRR) protects cells from a wild variety of DNA damage and it is regulated by RAD18 gene. Indeed, RAD18 deficient human cells exhibit sensitivity to ionizing radiation, suggesting a functional role of PRR for protection against ionizing radiation. Translesion DNA synthesis (TLS) is one of sub-pathway of PRR, in which a number of non-essential DNA polymerases is recruited to the 3'-ends and extends it beyond the lesions, rescues stalled replication. In eukaryotes, RAD6-RAD18 dependent mono-ubiquitination at the lysine 164 residue of proliferating cell nuclear antigen (PCNA) is deemed to play a key role in regulation of TLS. In this study, we demonstrated that the polymerase switching reactions were stimulated by mono-ubiquitination of PCNA.
