The Japan Radiation Research Society Annual Meeting Abstracts
The 53rd Annual Meeting of The Japan Radiation Research Society
Session ID : OA-3-3
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Host: The Japan Radiation Research Society

A. DNA damage and Repair
Evaluation of H2AX focus observed in the Ku80 deficient CHO mutant(xrs5)cells following BNCR (boron neutron capture reaction)
*Yuko KINASHI, Genro KASHINO, Ryuichi OKAYASU, Sentaro TAKAHASHI, Koji ONO
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Keywords: Boron Neutron Capture Reaction, DNA-double strand break, xrs5
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Abstract
Xrs5 (Ku80 deficient CHO mutant cells) are well-known for the DNA double strand breaks (DSBs) repair deficiency. The amount of DNA-DSBs following BNCR in the xrs5 cells was evaluated phosphorylated H2AX and 53Bp1 foci using the immunofluorescence intensity. Two hours after neutron irradiation, the number of H2AX and 53BP1 foci in the CHO-K1 cells was decreased to 20% of those at the 30min after irradiation. On the other hand, 70-80% of the foci observed at 30 min after the irradiation was still remained at 2 hours post-irradiation. Our results suggested that the DNA-DSBs induced by BNCR were not well repaired in the Ku80 deficient cells.
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