Space experiment "Neuro Rad" at Kibo-report: Space environmental effects on human neuron cultured cell line, SK-N-SH

Abstract

Space environmental condition composed of micro gravity and space radiation, of which dose is at least one hundred times more compared with those on the earth. Long-term manned space mission inevitably receive space radiation exposure and microgravity might give a synergistic effect on those space radiation effect. Human SK-N-SH cell is a slow growing neuron like cells, of which p53 status is normal. We send the cells to International Space Station, Japan Experimental Module (JEM), namely "Kibo" by STS131(19A) and cells were cultured at 37˚C with 1G or μG condition for 14 and 28 days at CBEF. Then cells were fixed and stored at -80˚C. The cells were returned by STS132 (ULF4). The frozen samples were transported to "Tsukuba Space center", and then brought them to Kagoshima University. Now, gene expression analysis was started using the sample. Mitochondrial impairment will be also examined using the same samples.