Detection and stability evaluation of spin adducts of CYPMPO with water decomposition radicals

Abstract

The reactive oxygen species play crucial roles in biological oxidative damage. In order to clarify the behaviour of hydroxyl and superoxide radicals closely related to the indirect effect of radiation damage of living body and DNA, detection and quantification of these radicals are essential. These radicals are easily produced by radiolysis of water but short lived. In the previous work we reported the rate constants of the reaction of a novel spin trap CYPMPO (5-(2,2-dimethyl-1,3-propoxy cyclophosphoryl)-5-methyl-1-pyrroline N-oxide) towards water decomposed species, e^-_aq and OH radical. In the present study we have detected these radicals as spin adducts of CYPMPO by ESR and stability of these spin adducts, especially under the acid condition, have been evaluated.