Abstract
Radiotherapy improved the local control by the development of instrumentation, machine software and diagnostic techniques. Despite these technical improvements, the satisfactory results are not yet provided about the regulation of tumor cell migration and invasion. Recently, although it is reported that radiation accelerates cell motility, little is known about molecular mechanisms of radiation-induced cell migration and/or invasion. Hence, in this study, we investigated to clarify the molecular mechanisms of radiation-induced cell migration.
Human glioblastoma CGNH-PM and U251 cells were exposed to X-rays (200 kVp, 14.6 mA). Cell migration was evaluated by wound-healing assay 24 h after X-ray irradiation. Cell migration was accelerated by X-ray irradiation in dose-dependent manner. The amount of glutamate released in culture medium was also increased in dose-dependent manner. Antagonist of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) type glutamate receptor suppressed radiation-induced cell migration.
These results suggest that glutamate is involved in radiation-induced cell migration. A further investigation about correlation between cell adhesion molecule and X-ray-induced cell migration via glutamate is being analyzed.
