Abstract
We have been developing new assay systems that use GFP gene expression for mutant detection. The principle of the approach is to make the cells fluorescent when mutation occurs at specified gene loci in the genome. One example is to make partial duplications of endogenous gene, in which the distal segment of the duplicate has a GFP gene tag. In this system, reversion from the duplicate makes the mutant cells fluorescent. The other example is to introduce cells co-expression of two vectors, one produces GFP constitutively and the other produces repressor protein to silence the expression of GFP gene. In this system, any kinds of forward mutation in the repressor gene in the targeted allele allow GFP gene transcription from null state, which make the mutant cells green. Both of the knock-in mice were generated. We report the current status of the mouse analyses.
