Abstract
Background
It is believed that the main cause of carcinogenesis is the accumulation of DNA damage and mutations in oncogene or tumor suppressor gene. However the mutation frequency is too low to explain the cancer development. On the other hand, it is well known that there are some aneupoid cells in tumor cells, suggestion the involvement of aneuploidy on cancer development. However we don't know whether aneuploidy is main cause of carcinogenesis or not. Here we established the aneupoid cells in which a human chromosome has been introduced artificially, and analyzed the cells focused on the phenotypes of the carcinogenesis.
Material and Method
We used immortalized adult human mesenchymal stem cells, and established two aneproid cell lines by microcell-mediated chromosome transfer method (each contains exogeneous human chromosome #1 and #7). We used the cells for carcinogenesis containing only drug resistance gene as a control cell line. We examined that 1) cell growth ratio, 2) anchorage-independent growth 3) ability of the cell growth in transplant for a mouse for index of the carcinogenesis in these cell lines. And we analyzed that 1) the abnormality of chromosome number, 2) structural abnormality of the chromosome and 3) micronuclei yields in aneuploidy cells for index of the chromosome instability. We examine the chromosome instability and carcinogenesis for aneuploidy cells in which a human chromosome #1 and #7 induced.
Results
The aneuploid cells which was #1 and #7 induced expressed characteristic of carcinogenesis. Though the cells induced #1 chromosome were redaction of the rate of cell growth, the anchorage-independent growth were 2-fold higher than tha in the control and #7 induced cells.
