Standardization of Spatial Resolution of Analytical Optical Microscopy

Abstract

Spatial resolution is one of the main specifications of confocal microscope. However, the definition and the measurement procedures largely vary depending on the manufacturer of the confocal Raman and confocal fluorescence microscope, therefore the general assessment of the spatial resolution has been limited. We try to provide standardized protocols that describe the measurement of the spatial resolution of analytical confocal microscopes by using small object method.