Study on physical properties measurement of minute bio-material (Part 1)

Measuring the force constant of the AFM micro-cantilever

Abstract

A setup to measure force constant of the atomic force microscope (AFM) micro-cantilever was developed for the purpose of measuring physical properties of minute size bio-material by AFM. Laser Raman spectroscopy enabled roughly detecting stress of bending micro-cantilever. By using piezo actuator and a micro-balance, it bacame possible to measure force constant of micro-cantilever in high precision. The measured results will be applied for the quantitative viscoelasticity measurement of minute size bio-material.