Studies on Hybrid Artificial Liver

-Metabolic Activity of PAN-HF module Seading Isolated Hepatocytes-

Abstract

The present experiment was disigned to evaluate metabolic abilities of a prototype for a hybrid artificial liver. The module utilizing polyacrylonitrile hollow fiber (Asahi Medical Co., Tokyo.) was employed for the prototype. The hepatocytes which were obtained from dog were sealed into the perfusate site of the module. The metabolic activity of the module was examined by an invitro perfusion technique. The following results were obtained that oxygen was cunsumed, ammonia decreased remarkably, urea-N and glucose increased slightly, and some amino asids changed in each individual range. These support the usefulness of the module for developing a hybrid artificial liver.