Experimental study of cryopreserved vascular homograft

Abstract

Cryopreserved vessels were experimentally examined. Canine caratid arteries and jugular veins were cryopreserved in dimethyl sulfoxide (DMSO) or in physiologic salt solution (PSS), quality of which were evaluated macroscopiaclly, microscopically, by scanning electron microscope and by measurement of compliance. Then cryopreserved canine carotid arteries and jugular veins in DMSO were grafted to bilateral canine carotid arteries and were removed at any proper days in 270 days from 3 days after the operation in order to examine macroscopically, microscopically and by scanning electron microscope. In conclusion, by use of cell cryoprotective agent DMSO, satisfactory grafts were obtained. The results of these experiments revealed that cryopreserved vessels in DMSO were excellent as small arterial prosthesis.