体外循環による血中α-Fetoprotein除去装置の開発

金井 弘一; 中島 猛行; 森岡 明; 賀古 真; 伊藤 龍彦; 姜 裕; 杉浦 康仁; 西脇 由朗; 水野 義仁; 礒田 治夫; 志知 泉; 早田 謙一; 寺村 淳; 外山 雅子; 渡部 博之; 佐藤 昇; 清田 隆夫; 林 紘

doi:10.11392/jsao1972.11.855

An extracorporeal circulation system for AFP removal from sera and its application for the treatment of hepatoma-bearing rats

K. KANAI, T. NAKAJIMA, S. MORIOKA, M. KAKO, T. ITO, Y. KAN, Y. SUGIURA, Y. NISHIWAKI, Y. MIZUNO, H. ISODA, I. SHICHI, K. SODA, J. TERAMURA, M. TOYAMA, H. WATABE, N. SATO, T. KIYOTA, H. HAYASHI

Author information

K. KANAI
Second Dept. of Med., University of Hamamatsu School of Medicine
T. NAKAJIMA
Second Dept. of Med., University of Hamamatsu School of Medicine
S. MORIOKA
Second Dept. of Med., University of Hamamatsu School of Medicine
M. KAKO
Second Dept. of Med., University of Hamamatsu School of Medicine
T. ITO
Second Dept. of Med., University of Hamamatsu School of Medicine
Y. KAN
Second Dept. of Med., University of Hamamatsu School of Medicine
Y. SUGIURA
Second Dept. of Med., University of Hamamatsu School of Medicine
Y. NISHIWAKI
Second Dept. of Med., University of Hamamatsu School of Medicine
Y. MIZUNO
Second Dept. of Med., University of Hamamatsu School of Medicine
H. ISODA
Second Dept. of Med., University of Hamamatsu School of Medicine
I. SHICHI
Second Dept. of Med., University of Hamamatsu School of Medicine
K. SODA
Second Dept. of Med., University of Hamamatsu School of Medicine
J. TERAMURA
Second Dept. of Med., University of Hamamatsu School of Medicine
M. TOYAMA
Second Dept. of Med., University of Hamamatsu School of Medicine
H. WATABE
Dept. of Biochem., Higashi-Nippon-Gakuen University
N. SATO
Inst. for Technol. Res., Asahi Chemical Industry
T. KIYOTA
Inst. for Technol. Res., Asahi Chemical Industry
H. HAYASHI
Inst. for Technol. Res., Asahi Chemical Industry

JOURNAL FREE ACCESS

1982 Volume 11 Issue 4 Pages 855-858

DOI https://doi.org/10.11392/jsao1972.11.855

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Abstract

An extracorporeal circulation system for removing α-fetoprotein (AFP) from rat blood was developed. The circulation system consisted of plasma separator, anti-AFP affinity column and blood pumps. In in vitro experiment where 30ml of fresh rat blood containing AFP was used, 70% of AFP was removed from blood during 4 hours of circulation. AFP was the only protein retained on affinity column. No changes in serum protein profiles other than AFP, was observed. Also, in in vivo experiment, AFP was selectively removed from the blood of hepatomabearing rats, although the decrease of serum AFP was less than that in in vitro probably reflecting the rapid growth of tumor cells in hepatoma rats. There was no differences in the size of tumor between experimental and control groups. However, the survival days of the rats treated by anti-AFP affinity column was longer (123%) than that of controls. Rats tolerated the circulation procedure well and no remarkable side effect was observed.

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