Abstract
A new method to give an ant ithrombogenicity to biological materials was developed. The method is as follows. A biological material such as a collagenous material was dipped into a solution of protamin sulphate The protamin adsorbed to collagen was crosslinked to the material by glutaraldehyde. Then, the protamin crosslinked material was dipped into a heparin solution (pH2.0). By this procedure, the heparin is sonically bound to the material. The amount and the distribution of the bound heparin can be controlled by changing the protamin fixation to the material. This method is expected to be used as an antithrombogenic method of artificial organs, and as a production method of anti adhesive membrane.
