Abstract
We studied the role of ligand (sulfathiazole), spacer (diamine corn-pounds) and pore size (300-2400Å) to the adsorption of plasma proteins in the preparation of chemically modified silica gel beads for immunotherapy. With an increase in surface area, the adsorption of albumin, globulin, IgG and IgA showed a tendency to increase. IgM and aggregated human IgG of high molecular weights, however, had the reverse tendency. The adsorption of albumin increased to some extent with the length of the ligand-spacer. Moderate length spacers indicated the highest value in the selective adsorption of IgM. Consequently, we found that the selectivity of adsorption was affected by the molecular sieving effect as well as the specific characteristic of the ligand. And it seems that an adsorbent having a moderate ligand-spacer and pore size is useful for the removal of higher molecule immunoglobulins in therapy.
