“APPLICATION OF OLIGOSACCHARIDE-CONTAINING POLYSTYRENE TO BIOSIMULATOR OF HEPATIC REACTION.”

Abstract

In this study, various oligosaccharide-containig polystyrene was synthesized and evaluated for the hepatocytes culture substrata. The highest adhesion of hepatocytes was attained with galactose containing polystyrene (PVLA). Thereafter, some cells were detached slowly, but significant portions of cells still survived even after 5 days' incubation. The binding of rat hepatocytes to PVLA required Ca²⁺. The adherent hepatocytes to PVLA (5 days) were detached with EDTA. The detached cells still have the functions and could spread in collagen-coated dishes.