Abstract
The purpose of this experimental study is to evaluate the histocompatibility of HA for cartilage tissue in vitro (in a further strict condition without inflammation). HA plates were prepared for organ culture. Cartilaginous femura were taken from 9 day chicken embryos and were placed on the HA plates, and in BGJb F-T Mod. medium supplemented with 10% chicken embryonal extract, PTH, and Vit. D3, incubated at 37°C in a humidified air with 5% CO2, and organ culture was maintained from 6 days to 8 days. By light microscopic examination, ingrowth of tissue of cartilage tissue of the embryo into HA pores was observed. And with the differentiation of the embryonal cartilage, ossification was observed at the site of embryonal femura adjacent to the HA plates. This in vivo study indicates a good histocompatibility of porous HA for cartilage tissue. And this organculture system model can be applied to evaluate the histocompatibility of ceramics materials for bone or cartilage.
