Abstract
An antiadhesive collagen membrane with heparin slow release was developed, using a new heparinization method by anchoring reagent Glycidyl Trimethyl Ammonium Chloride (GTMAC). This method is applicable to heparinize biological materilas without any crosslinking reagent. Heparin ionically bound to the materials released very slowly. GTMAC has two special groups, i. e., the epoxide group and the quarternary ammonium group. The epoxide group can react easily with amino groups, hydroxyl groups, and carboxyl groups of biomaterials. The quarternary ammonium group has positive charge, so that it can form a strong ionical complex with negative charged substances. Therefore, heparin can bind ionically to GTMAC which has bound to the biomaterials already. A fresh amnion membrane was heparinized using this method. The amount of heparin in the membrane was 15.5units/cmcm2. Two kinds of heparinized antiadhesive membrane were prepared. One was crosslinked by epoxy-compounds, another was non-crosslinked. As to the animal experiment, two 5-cm square pieces of the colonic serous membrane were removed from 20 dogs, and pieces of the heparinized membrane were sutured onto the sites. Adhesions were observed only to the sutures of the membrane in 4 cases, but no adhesion was on the membrane. Crosslinked membrane was not yet absorbed at 239 days after the surgery, but non-crosslinked membrane was already absorbed at 29 days. Both membranes showed antiadhesive property perfectly.
