Abstract
The hydrodynamic effect on clot growth and erythrocyte destruction was investigated quantitatively in vitro. Constant velocity gradient from 30 to 1, 500s-1 were applied to a canine blood sample contained in a cone and plate (made of polymethyl methacrylate) viscometer for 120min at 24°C. Evaluation of clot growth was derived from the clotting ratio (the volumetric fraction of clot in the whole blood), which was experimentally determined from the rate of increase of frictional torque between the rotating cone and the stationary plate. Evaluation of erythrocyte destruction was derived from the hemolysis ratio (plasma hemoglobin count per whole blood hemoglobin count). The results show that both the clotting ratio and the hemolysis ratio are maintained in small values (<0.3 and <0.01, respectively) for the velocity applying time of <120min at a velocity gradient from 400 to 1, 000s-1.
