Observation of leukocytes change during extracorporeal circulation using rabbit ear chamber technique

Abstract

It is well known that the number of peripheral leucocytes decrease during hemodialysis especially when cellulose membranes are used. This time, we observed microscopic leucocytes change during extracorporeal circulation using rabbit ear chamber technique. Leucocytes are observed rolling along the vascular wall slowly. After starting extracorporeal circulation, the leucocyte disappear in the visual field. Two or tree hours later, many leucocytes appear again at once. The change of peripheral leucocytes count correlates this phenomenon. At least, in this experience, the Craddock's theory, which explains the hemodialysis leucopenia resulting from the leucocyte's aggregation in the capillary, could not be proved.