Abstract
Hemoglobinuria and hemolysis combine with the cases under longtime extracorporal circulation of cardiac surgery. For the treatment of hemoglobinuria, it is necessary that quantitative analysis of free hemoglobin is performed rapidly and simply. Column method is simplified quantitative analysis by means of sepharose immobilized haptoglobin. This method was evaluated in 20 cases (9-valve replacement, 2-valve plasty, 9-CABG) under longtime extracorporal circulation and compared with ordinary method. The blood sample was examined immediately after stopping of extracorporal cerculation. In results, free hemoglobin was 49.1 31.7mg/dl by column method and 42.6 29.2mg/dl by ordinary method respectively. The results suggest that column method is useful analysis of free hemoglobin for the patients with hemoglobinuria as well as ordinary method.
