STUDY ON NEUTROPHIL FUNCTION & HISTOLOGY OF LUNG DURING CARDIOPULMONARY BYPASS WITH BUBBLE & MEMBRANE OCYGENATORS

Abstract

Post perfusion syndrome sometimes occurs after cardiopulmonary bypass. It is reported that pulmonary leukocyte sequestration and complement activation play a causative role to induce the syndrome. The purpose of this study is to compare bubble oxygenators with membrane oxygenators effecting on neutrophil function, complement activation and pulmonary leukocyte sequestration. Patient were divided into two groups. 10 patients were perfusate with bubble oxygenators (Group B) and 20 patients were perfusate with membrane ooxygenators (Group M). Number, H₂O₂ generation and agglutination of neutrophil, and serum complement activation (C3a, C5a)were measured. Lung biopy was performed in 5 of Group B and 10 of Group M before and after cardio ulmonary bypass and the number of neutrophil in the specimen from lung biopy were counted under light microscope. The results were as follows:
1. In Group B there were more increased neutrophil number, H202 generation during cardiopulmonary bypass and the increasing ratio of serum C3a between pre and post cardiopulmonary bypass as compares to those in Group M.
2. In Group B there were more number of agglutinative neutrophiles in the pulmonary tissue than those in Group M. These findings may suggest that membrane oxygenators is more physiological apparatous than bubble oxygenators.