1989 Volume 18 Issue 3 Pages 1155-1158
To search for macromolecular ligands strongly interacting with β2-microglobulin (β2-MG) we drew 3-dimentional structure of human β2-MG by computer graphics (C. G) and evaluated macromolecular ligands presumed from the results by C. G with a newly developed EIA plate method. In result, C. G demonstrated three regions on the molecular surface of β2-MG, the first rich in hydrophobic amino acid residues, the second rich in hydrophobic and basic amino acid residues, and the third rich in acidic ones. Methylated atelocollagen and stylene-maleic acid copolymer were selected by the EIA method. The molecular structure of these ligands was showed to complement well with thesurface structure of β2-MG drawn by C. G. The results indicate that the EIA plate method may be of use as an easy method of searching for macromolecular ligands and that an adsorbent for removing β2-MG in a very high efficiency can be made by conjugating selected ligands on porous particles.
