ADSORPTIVE CHARACTERISTICS OF PAN AND PMMA MEMBRANES FOR PROTEINS

Abstract

We examined the adsorptive characteristics of PAN and PMMA membranes for proteins. The membranes used for hemodyalysis were perfused with 8M ureic solution. And the perfusates were analyzed with two-dimensional electrophoresis(2-DE). In addition, removal proportion and sieving coeff cient (SC) of retinol-binding protein (RBP), lysozyme and β₂-microglobulin(β₂-MG) were examined in each membrane. High removal proportion and low SC of lysozyme suggested the strong adsorption in PAN membrane. 2-DE of PAN membrane perfusate showed many spots including β₂-MG, albumin (Alb) and transfferin(Tf)on polyacrylamide gel. PMMA membrane showed low SC of β₂-MG and RBP, which were recognized as spots on polyacrylamide gel as same as IgG and other proteins by 2-DE. These results showed the strong adsorption of β₂-MG and RBP in PMMA membrane. We concluded that adsorptive characteristics of PAN membrane were different from those of PMMA membrane for proteins.