Abstract
To evaluate the leucocyte and platelet activity during hemodialysis treatment, we assessed the white blood cell and its differential count, platelet count, β-thromboglobulin (β-TG), platelet factor-4 (PF-4), thromboxane B2 (TXB2) and the nitroblue tetrazolium reduction ratio of neutrophil. In addition, lipids peroxidation products (MDA) were measured to estimate the influence of free radicals during HD. Transient and significant Leucocytopenia and thrombocytopenia were found. The number of neutrophil transiently decreased at the early phase of HD, but lymphocyte counts were stable during HD. PF-4, β-TG and TXB2, released during platelet activation, were not found to have increased any, throughout the 4 hours after initiation of dialysis, compared with those made at the beginning. NBT reduction ratio of neutrophil was significantly reduced at 15 minutes after initiation of HD. Serum NDA levels were not significantly changed throughout HD. These results suggested that the activation of neutrophil and platelet during HD was not apparent, and free radical generation during one period of HD did not influence the producing of lipid peroxidation.
