Abstract
An important facet of this study is to develop new polymeric adsorbent with specific affinity toward a particular subpopulations of lymphocytes. Based on our strategy of separating lymphocyte subpopulations through their differential ionic affinity toward solid-phase matrices, a series of poly(2-hydroxyethyl methacrylate)/polyamine graft copolymers (HA copolymers) was prepared. HA copolymer columns were found to show specific adsorption affinity toward B cells out of a mixture of B and T cells. Further, a partially quaternized HA copolymer (HQA) was found to have a capability of resoluting even T cell subsets, indicating its utility as a novel adsorbent for T cell subsets.
