Abstract
Artificial Red Cell (ARC) was prepared by encapsulation of purified hemoglobin (Hb) with phospholipid mixtures, mainly polymerizable 1, 2-bis(2, 4-octadecadienoyl)-sn-3-phosphocholine (DODPC), and it was subsequently stabilized by polymerization. Concerning physical characteristics, particle diameter 0.2μm, [Hb] 7-10g/dl, osmotic pressure 300mOsm, oncotic pressure 25torr, viscosity 4cp. P50 was adjusted to 30-40torr. Concerning physical stability, ARC showed no change in particle size and size distribution and no leakage of Hb after repeating freeze-thawing treatment. In the blood compatible tests (in vitro), there was no difference between ARC and control (PBS) about blood coagulation time [TT, PT, APTT, platlet aggregation], red cell hemolysis and red cell aggregation.
