Abstract
PHP containing UW components were evaluated as perfusates for normothermic preservation of the isolated dog kidney. Kidneys flushed out by Lactate Ringer solution were connected to the perfusion circuit which consists of a preservation box, a reservoir of perfusate, a membrane oxygenator and a driving unit. PHP containing 140mEg/l of Na+ and 4mEq/l of K+(PHP(E)), UW solution (UW) and UW components added PHP(E) were adjusted at pH 7.4 prior to use. During 12 hour perfusion at 37°C with perfusion pressure of 100mmHg, remarkable change in pH were observed in UW and PHP(E) while higher oxygen consumption was noted in PHP(E)+UW than in PHP(E). The histological findings showed moderate damages of tubular epithelial cells and containing normal glomelular structure in PHP(E)+UW. There was no edematous degeneration in both UW and PHP (E)+UW, however, it was seen in PHP(E) alone. It was suggested that components of UW solution have positive effects on normothermic machine perfusion with PHP(E) solution.
