DESIGN AND EVALUATION OF ASIALOGLYCOPROTEIN MODEL POLYMER FOR REGULATING DIFFERENTIATION AND PROLIFERA-TION OF HEPATOCYTES

Abstract

We found that regulating of differentiation and proliferation of hepatocytes which were cultured on polystyrene dishes can be achieved by varying the amount of lactose-carrying styrene polymer (PVLA) coated on the dishes. The result of the experiment, 3H-thymidine up-take of cultured hepatocytes is the lowest for cells cultured on dishes coated with high amount of PVLA in which the hepatocytes are round and high bile acid release was observed. But the highest thymidine uptake was observed in dishes with low PVLA coating in which the hepatocytes are spread and low bile acid release was observed.