Abstract
The object of present study is the development of a continuously monitoring sensor for ET concentration in dialysate. ET is readily capable of penetrating from dialysate into blood through highly permeable membranes. Dialysate should be kept ET free and should be observed absence of ET by continuous measurement. Limulus test is generally used for the measurement of ET concentration in clinical settings. Conventional operation of Limulus test needs complicated operation because of its sensitivity to contamination from atmosphere. Therefore conventional Limulus test is unsuitable for continuous ET measurement. The authors designed a new ET sensor operating the continuous reaction of Limulus test in single tube. This sensor enabled sample solution to react without contact with atmosphere and simplified continuous ET concentration measurement. The sensor showed faster response than conventional Limulus test and had measurable ET concentration range concluding allowable ET concentration limit. The developed ET sensor is promising for monitoring of ET in dialysate lines.
