Abstract
Effects of hemodialysate and plasma of 11 patients with chronic renal failure (CRF) on lymphocyte blastogenesis was examined. Of 11 hemodialysate 10 enhanced [3H]thymidine incorporation into mitogen-stimulated healthy lympocytes. Plasma of these patients before dialysis significantly enhanced lympocyte blastogenesis when compared to healthy plasma (n=10), whereas the activity of plasma after dialysis did not differ significantly from that of healthy plasma. The experimental hemodialysate of CRF blood significantly enhanced lymphocyte blastogenesis, when compared to that of healthy blood. Healthy or CRF plasma was filtrated by a membrane with a molecular cut off of 3, 000 daltons. The filtrate of CRF plasma significantly enhanced blastogenesis, compared to that of healthy plasma. This activity did not abolish by heat tratment (100°C, 40min). Any drugs administered to the patients or creatinine did not enhance blastogenesis. These results suggest the presence of blastogenesis enhancing factor(s) in CRF hemodialysate.
