Abstract
Our previous report showed that albumin conjugated with the peptide, Gly-Arg-Gly-Asp-Ser (GRGDS) which contains RGD tripeptide sequence identified as the minimum core cell adhesive peptide, exhibited cellular adhesive responses (adhesion, spreading and migration) similar to those of fibronectin and vitronectin. In this paper, we determine the interaction strength between cell receptor-ligand from the dosedependent inhibitory response by addition of GRGDS into a medium. The result showed that the half-inhibition concentration of endothelial cell adhesion on GRGRDS-conjugated albumin was much lower than those on vitronectin and fibronectin. Little appreciable change was observed when the peptides identified as accessory adhesive sites of fibronectin were derivatized on albumin. This indicates that, as compared with natural counterparts, GRGDS-conjugated albumin interacts quite weakly with endothelial cells.
