Abstract
The regulation of the oxygen affinity with allosteric effectors and the dehydration, in the presence of saccharides, of the hemoglobin vesicle (abbreviated as ARC) that was obtained by encapsulating the purified Hb with polymerizable phospholipids, were investigated. The oxygen affinity of ARC can be modulated to the same level as that of red blood cell (P50=27.0mmHg, OTE=25.0%, n=2.5) by coencapsulating demand amount of pyridoxal-5-phosphate or 2, 3-diphosphoglycerate and chloride ion. Furthermore, the dried ARC, which was dehydrated in the presence of saccharides, can be recovered by dispersion of the powdered ARC in water. The change in the particle size, the leak of the interior Hb and the increase in the metHb percentage were not observed. The oxygen affinity was also maintained unchanged.
