Abstract
In this study, we measured the platelet concentration of calcium ions (Ca2+) and thrombin-stimulated platelet Ca2+ increases during hemodialysis in 33 patients (19 male, 14 female, mean age 55.2±2.3 years) with chronic ronal failire. The following results were obtained:1)Just after hemodialysis with a semi-synthetic membrane dialyzer, platelet Ca2+ increased significantly. However, this did not occur during hemodialysis with a synthetic menbrane dializer or during extra-corporeal ultrafiltration with both menbrane dialyzers. 2)The increase in platelet Ca2+ by thrombin stimulation at a dose of 0.1U/ml was significantly larger after than before use of the semi-synthetic dialyzer. This phenomenon was not seen during hemodialysis with the synthetic menbralne dialyzer. 3)Platelet Ca2+ decreased significantly just after the injection of anti-coagulants (heparine or nafamostat mesilate). 4)The anti-coagulants significantly suppressed the thrombin-induced increase in platelet Ca2+. The mobilization of Ca2+ in platelets and the influx of Ca2+ into platelets both decreased significantly after anti-coagulants adnministration. These results suggest that platelet Ca2+ concentration tends to increase after blood passed through a semi-synthetic membrane dializer, and suppress increases in platelet Ca2+, induced by thrombin stimulation.
