DEVELOPMENT OF FERROCENE-MEDIATED NEEDLE-TYPE GLUCOSE SENSOR COVERED WITH MPG MEMBRANE

—EXAMINATION OF BIOCOMPATIBILITY OF MPG MEMBRANE—

Abstract

To prepare a long-life and stable glucose sensor, a newly designed biocompatible mem-brane, consisting of 2-methacryloyloxyethyl phosphorylcholine (MPG), has been developed and applied to the surace of a ferrocene-mediated needle-type glucose sensor. In vitro experiments, the amounts of adsorbed protein on PVA or MPG membrane were 35.6, 14.0μg/cm² on 14th day, respectively. In vivo experiments, the amounts of adsorbed protean on PVA or MPG membrane were 31.2, 16.9μg/cm² on 14th day, respectively. Therefore, we conclude that MPG membrane exhibited exceller biocompatibility in vitro and in vivo. We are now applying wearable artificial endocrine pancreas with this sensor to long-term clinical treatment of ambulatory diabetic patients.