Abstract
We investigated the binding kinetics of charged drugs by dialysis membranes via an ionic bond in bovine serum. Four positively charged drugs, that is, nafamostat mesilate, cimetidine, procainamide, diltiazem, and four negatively charged drugs, that is, heparin, furosemide, cefotaxime, and carbazochrom were studied. Two dialysis membranes, that is, polyacrylonitrile (AN69) as a negatively charged membrane and hemophan (HE) as positively charged membrane were cut into small fibers and incubated in bovine serum prior to the adsorption test. Each drug also incubated in the bovine serum, and then dialysis membranes and drugs were combined. The adsorption of cimetidine and diltiazem to AN69 membrane were weak in the serum in comparison to the binding capacity in the water. On the other hand, the adsorption dose of nafamostat mesilate and procainamide were not different between the adsorption test in the water and serum. The adsorption of carbazochrom to HE membrane was weak in the serum. Heparin, furosemide, and cefotaxime were adsorped equally by HE membrane in the water and serum. These results suggest that each charged drug is adsorped by the dialysis membrane in the different manner for the protein binding of drugs and dialysis membranes.
