Abstract
Cryofiltration (CRYO) is the system of protein adsorption using heparin. Under low temperature and adding of heparin, cryogel is produced from the plasma of patients by on-line system. The produced cryogel, however, narrow and obstruct pores of the filter. Therefore, the second filter has to be irrigated to remove the produced cryogel regularly. And device of this washing method is very important because it decides the size and volume of the removing pathological proteins. In this study, we devised two types of washing method. One was A type: 300mmHg of perfusion pressure, 200ml of replacement saline and 300ml of washing saline. B type was 400mmHg of pressure, no replacement and 50ml of washing saline. Reduction rate of pathologic proteins and physiologic influence of patients by washing methods were observed. EDA (+) Fibronectin (one of pathological protein) was reduced to 70.2±2.3% by A type and 81.6±2.0% by B type after CRYO. 340ml of body fluid was increased by A type and 490ml was reduced by B type. As a result, 100% of efficiency of A type was corresponded to 70% of B type. This 30% of a deduction will be cut the perfusion time. And physiologically, B type was better than A type.
