Abstract
In this study, we investigated about active oxygen and morophological change of HL-60 cells adherated polymeric materials. Active oxygen produced by neutrophil-like HL-60 cells on polymeric materials was measured with chemiluminescence detector. The levels of active oxygen produced by cells on various polymer were different. However, there was no correlation between active oxygen and contact angle. Polystyrene was then sulfonated with concentrated sulfuric acid As the degree of sulfonation increased, the active oxygen produced by the cells decreased The cell adhesion rates on polystyrene sulfonated polystyrene were not different. The fine structures of neutrophil-like HL-60 cells adhering to modified polystyrene were observed by scanning electromicroscopy. The shape of cells on polystyrene was flat, whereas it was normal on sulfonated polystyrene. The fluorescence photobleaching recoveries on cell membrane were different between the two polymeric surfaces.
