Abstract
A stopped flow type system consisting of a single tube for monitoring endotoxin by the limulus test was developed. In this system sample solution reacted with limulus reagent without contact with outer atmosphere. The objective of the present study is to obtain a sensitive system for monitoring endotoxin concentration ranging under 100 EU/l in a short time. Peak area of absorbance observed as a function of reaction time at 405 nm was similar to absorbance observed with batch reaction. Short time monitoring of endotoxin concentration ranging above 40 EU/l at a reaction time of 20 min and 80 EU/l at 10min was possible.
